Effects of Recombinant EPO on Death of Cortical Neuron inChronic Hypoxia (만성 저산소 환경에 의한 신경세포의 사멸에 recombinanterythropoietin이 미치는 영향)

김호정; 백진기; 양성준; 강기영; 김강련; Hyun Kim (김현)

doi:10.11637/aba.2005.18.4.291

Effects of Recombinant EPO on Death of Cortical Neuron inChronic Hypoxia

Anatomy & Biological Anthropology
Abbr : Anat Biol Anthropol
2005, 18(4), pp.291~301
DOI : 10.11637/aba.2005.18.4.291
Publisher : 대한체질인류학회
Research Area : Medicine and Pharmacy > Anatomy

김호정 ¹, 백진기 ², 양성준 ², 강기영 ², 김강련 ², Hyun Kim ³

¹관동대학교
²한양대학교
³부산대학교

Candidate

ABSTRACT

Chronic hypoxia has been associated with change in neurovascular behavior, mediated, in part, by erythropoietin (EPO). EPO, a hematopoietic growth factor, could act as a neurotrophic factor. In the present study, we investigated the characteristics of EPO and erythropoietin receptor (EPOR) expressions by cortical neuron in vivo and in vitro and tested the hypothesis that EPO serves protective functions under chronic hypoxia. E18, P5 and P7 mice for 3 days and primary cultured neurons for 6 days were incubated in hypoxic conditions consisted of a mixture of 10% O2, 5% CO2, 85% N2. To study expressions of EPO, EPOR, caspases, pAKT, pERK, and PARP, immunohistochemical stainning and western blotting were carried out. In addition to expressing EPO and EPOR under normoxic conditions, neurons increased their expression of EPO and EPOR under hypoxia. The effects of recombinant EPO appeared to be mediated via the phosphatidylinositol (PI) 3-kinase-AKT pathway, correlated directly with activation of caspase 3. Also recombinant EPO decreased expression of caspase 8, but not caspase 9. Finally, recombinant EPO decreased apoptosis of cultured neurons as evaluated by expression of PARP. These data support a role for EPO in maintenance of cortical neuron under chronic hypoxia.

KEYWORDS

Chronic hypoxia, Neuronal cell death, Recombinant EPO, EPOR

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Anatomy & Biological Anthropology 2023 KCI Impact Factor : 0.33

Effects of Recombinant EPO on Death of Cortical Neuron inChronic Hypoxia

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Anatomy & Biological Anthropology 2023 KCI Impact Factor : 0.33

Effects of Recombinant EPO on Death of Cortical Neuron inChronic Hypoxia

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