2007, Vol.17, No.4

Journal of Korean Medicine Rehabilitation 2023 KCI Impact Factor : 0.6

pISSN : 1229-1854
https://journal.kci.go.kr/jkmr

Objectives : This study was performed to investigate the effects of Yukmijiwhang-tang (Liuweidihuang-tang) with Puerariae raidx(YMG) on the ovariectomized rat model of postmenopausal osteoporosis. Methods : We used 9 week aged Sprague-Dawley rat and divided into four group, sham operation group(Control, C), ovariectomized group(Negative Control, N.C), administrated YMG 1,250 mg/kg/day after ovariectomy group(T1), administrated YMG 2,500 mg/kg/day after ovariectomy group(T2). Then we observed changes in the body weight, body fat, serum metabolic products, bone density and so on. Results : 1. Body weight in negative control group showed significant increase in comparison with control group, but that in T1 and T2 group showed decrease in comparison with negative control group. 2. In the autopsy, negative control group showed uterus atrophy in comparison with control group. While T1 and T2 group showed decrease in uterus atrophy in comparison with negative control group. 3. In the total cholesterol, negative control group showed a significant increase in comparison with control group. While T1 and T2 group showed a significant decrease in comparison with negative control group. 4. In the body fat, negative control group showed a tendency to increase in comparison with control group. While T1 and T2 group showed a tendency to decrease in comparison with negative control group. 5. In bone density, normal control group showed a tendency to decrease bone density in comparison with control group. While T1 and T2 group showed a tendency to increase bone density in comparison with negative control group. Conclusions : YMG has shown to be capable of preventing and curing osteoporosis induced by ovariectomy osteoporosis rats.

Objectives： This study was carried out to investigate the effects of low frequency 2 Hz electroacupuncture(EA) on the paw edema and phosphorylation of spinal N-methyl-D-aspartate(NMDA) receptor. Methods： Inflammation was induced by an intraplantar injection of 1% carrageenan into the right hind paw. Bilateral EA stimulation with 2 Hz were delivered at those acupoints corresponding to human Zusanli(S36) and Sanyinjiao(Sp6) via the needles for a total of 30min duration. Results： 1. The 2 Hz EA stimulation presented significant edema inhibition with reduced degranulation of mast cells in the paw connective tissue and this stimulation also reduced the development of both thermal and mechanical hyperalgesia. 2. The number of c-fos immunoreactive neurons was significantly increased in the superficial laminae and nucleus proprius of the dorsal horn at L4-5 spinal segment by carrageenan injection. But the immunoreactive neurons in superficial laminae of the dorsal horn markedly reduced by EA stimulation. 3. The results of Western blot showed that the level of NR1 subunit, phoshphorylated NR1 and NR2B subunits was induced by carrageenan injection, but there were no significant changes by EA stimulation. 4. In immunohistochemical analysis, the phosphorylated NR2B immunoreaction also expressed highly in the superficial laminae of spinal cord by carrageenan injection. But the phosphorylated NR2B subunit was suppressed by EA stimulation. Conclusions： These results indicate that low frequency EA stimulation have an alleviating function against carrageenan-induced edema and hyperalgesia pain through modulating NMDA receptors and its phosphorylation.

Objectives : This study was designed to evaluate the effects of Jeho-tang(Tihutang) and Ganoderma Lucidum extract on the recovery of fatigue induced by treadmill exercise in rats. Methods : The rat subjects were divided into 4 groups : Normal, Control with treadmill exercise under 70% max(VO2max) intensity, Experimental Ⅰ(Ex Ⅰ) taken with Jeho-tang during treadmill exercise and Experimental Ⅱ(Ex Ⅱ) taken with Ganoderma Lucidum extract during treadmill exercise. All the serum samples collected by heart puncture were used to measure biomechanical factors such as lactate dehydrogenase(LDH), ammonia, inorganic phosphate, creatine kinase(CK), glucose, lactate, triglycerides(TG), high-density lipoprotein(HDL), low-density lipoprotein(LDL) and total cholesterol(TC). Besides, glycogen concentration and Heat shock protein 70(HSP70) by western blotting were measured. Histopathological changes in liver, kidney and muscle were investigated. Results : 1. LDH, ammonia, inorganic phosphate and lactate were significantly decreased in the experimental groups as compared with control group(p<0.05). 2. TC and LDL were significantly decreased in the Ex Ⅰ and Ex Ⅱ as compared with control group(p<0.05). 3. The HSP70 expression was increased in all the groups, especially significant in Ex Ⅱ. Conclusions : According to the above results, Jeho-tang(Tihutang) and Ganoderma Lucidum extract can recover the fatigue after high intensity exercise. If Jeho-tang (Tihutang) and Ganoderma Lucidum extract can be more useful as ergogenic aids, further studies about their underlying mechanism may be needed.