Objectives: This study aimed to verify the degree of clinical practice transition shock, resilience, and academic burnout of dental hygiene students, and analyze factors affecting academic burnout. Methods: From September 1 to September 20, 2022, this study collected data from 201 dental hygiene students who experienced clinical practice, and subsequently conducted a t-test, one-way ANOVA, Pearson correlation analysis, and multiple regression analysis using the SPSS Statistics 22. Results: The transition shock and resilience showed a significantly negative correlation (r=-0.211), transition shock and academic burnout showed a significantly positive correlation (r=0.484), and resilience and academic burnout showed a significantly negative correlation (r=-0.568). Regarding factors affecting academic burnout, academic burnout increased when the transition shock was greater (β=0.263, p <0.001), major satisfaction after clinical practice was lower (β=0.180, p <0.05), and the average grades were lower (β=0.169, p <0.01). Academic burnout decreased when resilience was greater (β=-0.435, p <0.001). Conclusions: To prevent clinical practice transition shock and academic burnout, and to increase the resilience of dental hygiene students, it would be necessary to establish learning strategies, change the educational environment, and establish a systematic clinical practice management program.