Orostachys japonicus, a medicinal plant, is a perennial herbaceous plant that grows on rocks and tile roofs. It grows like a pine tree, so it is sometimes called a houseleek. Recently, the extract of Orostachys japonicus has been reported to have immune enhancement, anticancer effect, apoptosis inducing effect on leukemic cells, antioxidant effect, antibacterial effect, dietary anti-hyperlipidemic effect, anti-diabetic activity effect, and antimutagenic effect. The substances isolated by solvent extraction were known flavonoids, triterpenes, sterols, derivatives of quercetin and kaempferol. Protein peptide fractions were separated and analyzed for anti-inflammatory activity and antimicrobial activity. To confirm the cytotoxicity of the separated peptide 3 KDa fractions, Raw 264.7 cells were used as the macrophage cell line and the cell viability was 90% or more at a concentration of 1 μg/mL and a concentration of 10 μg/mL. As a result of measuring NO reduction rate, NO decreased about 81% at the concentration of 10 ug/mL of peptide 3 KDa fraction. The antimicrobial activity of the peptide fractions inhibited the growth of Escherichia coli, which is a gram negative bacterium, and Bacillus cereus and Bacillus subtilis, which are Gram positive bacteria, but did not exhibit strong antimicrobial activity as compared with control methanol. In the case of the products using the protein peptide, there is no research on the efficacy for the standardized manufacturing method and the origin of country. Therefore, functional studies are urgently required and this paper can be used as a basic data.