Objectives: This study aimed to improve first aid knowledge, emergency response skills, and training activation in dental hygienists by providing data associated with emergency management in dental clinics. Methods: Data from 178 dental hygienists in Gwangju, Jeonnam, and Jeonbuk were collected through a survey conducted between June and August in 2024; survey responses were analyzed using SPSS. Results: Our results revealed significant differences in first-aid confidence and emergency response abilities based on age, experience, education level, first-aid training, emergency situation exposure, and CPR certification. A strong positive correlation was observed between first-aid confidence and emergency response abilities (r=0.783, p<0.001). Key factors influencing first-aid response for systemic diseases were first-aid confidence (β=0.651, p<0.001), less than 4 years of experience (β=0.201, p<0.001), and educational experience (β=0.165, p=0.002). This indicates that greater confidence, shorter clinical experience, and prior education enhance response capabilities. Conclusions: This study found that confidence and education significantly affected emergency response skills. Regular hands-on training, diverse educational programs, and standardized first-aid manuals are essential for improving the preparedness, attitudes, and competencies of dental hygienists.