The purpose of this study was to investigate the effects of 7 days of Kinesio taping on muscle tone, stiffness, elasticity, ankle range of motion, and muscle strength in college soccer players with functional ankle instability. The results of this study showed that after Kinesio taping, the tibialis anterior muscle tone (p<.01), stiffness (p<.001), and elasticity (p<.05) all decreased from the 5th day, while the fibularis longus muscle tone (p<.01) significantly decreased from the 4th day, stiffness (p<.01) from the 6th day, and elasticity (p<.01) from the 5th day. The dorsiflexion (p<.01), plantarflexion (p<.01), and eversion (p<.05) ranges of motion all significantly increased, and the eversion strength (p<.05) significantly increased. Therefore, it is thought that it will have a positive effect on the functional ankle instability of soccer players, and it is recommended that Kinesio taping be applied continuously for at least 5 to 7 days to improve FAI of soccer players.